1. What is DNA ng to pmol Conversion?

The DNA ng to pmol conversion calculates the molar quantity of DNA from its mass, taking into account the length of the DNA molecule. This is essential for molecular biology applications where molar concentrations are required.

2. How Does the Calculator Work?

The calculator uses the following equation:

Where:

• \( ng \) — Mass of DNA in nanograms
• \( length \) — Length of DNA in base pairs
• \( 660 \) — Average molecular weight of one DNA base pair (in g/mol)
• \( 1000 \) — Conversion factor from ng to g

Explanation: The equation converts mass (ng) to molar quantity (pmol) by accounting for the length-dependent molecular weight of DNA.

3. Importance of DNA Quantification

Details: Accurate DNA quantification is crucial for PCR, sequencing, cloning, and other molecular biology techniques where precise molar amounts are required for optimal results.

4. Using the Calculator

Tips: Enter DNA amount in ng and length in base pairs. All values must be valid (ng > 0, length ≥ 1 bp).

5. Frequently Asked Questions (FAQ)

Q1: Why is 660 g/mol used for DNA base pairs?
A: This is the average molecular weight of a DNA base pair (dNTP), accounting for the different weights of A, T, C, and G.

Q2: Does this work for single-stranded DNA?
A: No, for ssDNA use 330 g/mol as the average molecular weight per nucleotide instead of 660 g/mol per base pair.

Q3: How accurate is this calculation?
A: It provides a good estimate for most applications, but actual molecular weight may vary slightly based on GC content.

Q4: What if my DNA is in µg instead of ng?
A: Convert µg to ng by multiplying by 1000 before using the calculator (1 µg = 1000 ng).

Q5: Can I use this for RNA quantification?
A: No, RNA calculations should use 340 g/mol as the average molecular weight per nucleotide.