1. What is ng/µL to nM Conversion?

The ng/µL to nM conversion calculates molar concentration (nM) from mass concentration (ng/µL) using the molecular weight of the substance. This is essential in molecular biology and biochemistry for comparing concentrations across different compounds.

2. How Does the Calculator Work?

The calculator uses the conversion formula:

Where:

• \( ng/µL \) — Mass concentration in nanograms per microliter
• \( MW \) — Molecular weight in grams per mole
• \( 1000 \) — Conversion factor (ng to µg and µL to mL)

Explanation: The formula converts mass concentration to molar concentration by accounting for the molecular weight of the substance.

3. Importance of Concentration Conversion

Details: Accurate conversion between mass and molar concentrations is crucial for preparing solutions, comparing studies, and ensuring proper reagent concentrations in experiments.

4. Using the Calculator

Tips: Enter concentration in ng/µL and molecular weight in g/mol. Both values must be positive numbers. The result will be in nM (nanomolar).

5. Frequently Asked Questions (FAQ)

Q1: Why convert ng/µL to nM?
A: Molar concentration (nM) allows comparison between different molecules based on number of molecules rather than mass, which is more meaningful in chemical reactions.

Q2: What if my molecular weight is in kDa?
A: Convert kDa to g/mol by multiplying by 1000 (1 kDa = 1000 g/mol) before entering the value.

Q3: How precise should my molecular weight be?
A: Use the exact molecular weight for precise calculations. For proteins, use the calculated MW from the amino acid sequence.

Q4: Can I convert nM back to ng/µL?
A: Yes, use the formula: \( ng/µL = (nM \times MW) / 1000 \).

Q5: Does this work for all molecules?
A: Yes, as long as you know the exact molecular weight of your compound (DNA, RNA, protein, small molecule, etc.).